Detection of fungal pathogens by a new broad range real-time PCR assay targeting the fungal ITS2 region

Clinical Utility of Broad-Range Fungal PCR

Detection of invasive fungal pathogens by real-time PCR and high-resolution melting analysis.

BACKGROUND/AIM Causative agents most frequently encountered in systemic infections are bacteria, although fungi that cause invasive infections have also emerged, mostly in immune-compromised patients. The early detection and adequate treatment of bloodstream infections are critical for successful treatment. The aim of this study was to develop a rapid and efficient method for the detection and ...

Fungal PCR Assays Operons for Development of Broad-Range Sequencing and Analysis of Fungal rRNA

Sequencing and analysis of fungal rRNA operons for development of broad-range fungal PCR assays.

rRNA genes are attractive targets for developing PCR assays targeting human fungal pathogens. Most studies have focused on the 18S rRNA gene, internal transcribed spacers, and the 5' end of the 28S rRNA gene. An approximately 2,900-bp region of the 28S rRNA gene remains largely unexplored because sequences of many medically relevant fungi are either unavailable or undefined in genomic databases...

Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay

Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...